Lopamudra Ray

Assistant Professor

Dr Lopamudra Ray obtained her PhD Degree from KIIT University in 2012. Her PhD topic was "Isolation, taxonomic characterization, partial purification of chitinases and application potential of novel Streptomyces strain from Chilika Lake". After completion of her PhD she joined as a research scientist in School of Biotechnology, KilT University. During her course of doctoral studies , Dr Ray looked for chitin degrading Streptomyces strains with novel properties. She had also visited Swiss Federal Inst itute of Environmental Sciences ( EA WAG), ETH and received exposure to various techniques i n the filed of environmental and molecular microbiology . She has qualified National Eligibility Test conducted by Indian Council of Agricultural Research 2005. • She also Worked as Post Doctoral Research Scientist (3Years) from 2013 to 2016 in a World Bank funded project on “Isolation and Characterization of Benthic as well as Littoral Microbial Flora of Chilika Lake sponsored by World bank in School of Biotech, KIIT. She is working as a Assistant Professor in School of Law since 2016. She teaches Recombinant DNA technology, Fundamentals of Biotechnology and Forensic Sciences to BSc LLB students and Microbiology to MSc Microbiology and MSc Biotechnology students in other schools.

Profile Links

Email :
[email protected]
Scopus Id :
https://www.scopus.com/authid/detail.uri?authorId=57196028639

Social Links

Research Interests
Environmental Microbiology, Bioprospecting, Enzyme Purification, Microbial Taxonomy

Projects
1. DBT Biocare (2017-2020) on "Production of chitin and chitosan oligomers by Novel Streptomyces strains isolated from Chilika lake, and their use as bio protective agent developing an economically viable process -[ 20052 ]" 2. IBSD/A1/P(PH-2)/1 :“Bio prospecting microbial diversity in benthic and littoral regions of Loktak Lake for effective management and sustainable development”
Journals/Conferences :
• Ray, L et al Streptomyces barkulensis sp. nov., a novel halophilic streptomycete isolated from an estuarine lake. DOI 10.1099/ijs.0.056614-0
• Ray, L et al. Streptomyces chilikensis sp. nov., a novel halophilic streptomycete isolated from brackish water sediment of Lake Chilika in Odisha DOI: 10.1099/ijs.0.046284-0
• Ray, L et al Streptomyces chitinivorans sp. nov., a chitinolytic strain isolated from estuarine lake sediment. DOI 10.1099/ijsem.0.001176
• Samir R. Mishra, Lopamudra Ray, A.N.Panda, Neha Sahu, Sonal S. Xess, Sudhir Jadhao, Mrutyunjay Suar, Tapan K Adhya, Gurdeep Rastogi, Ajit K. Pattnayak, Vishakha Raina Draft genome sequence of Acinetobacter sp. strain BMW17, a cellulytic and plant growth promoting bacterium isolated from the rhizospheric region of Phragmites karka of Chilika lake doi: 10.1128/genomeA.00395-16
• Samir R. Mishra, A.N. Panda, Lopamudra Ray, Neha Sahu, Gayatri Mishra, Sudhir Jadhao, Mrutyunjy Suar, Tapan K Adhya, Gurdeep Rastogi, Ajit K Pattnaik, Vishkha Raina Draft genome sequence of Pseudomonas sp. strain BMS12, a plant growth promoting and protease producing bacterium isolated from the rhizosphere sediment of Phragmites karka of Chilika lake,DOI: 10.1128/genomeA.00342-16
• Samir R. Mishra, A.N. Panda, Lopamudra Ray, Neha Sahu, Gayatri Mishra, Sudhir Jadhao, Mrutyunjy Suar, Tapan K Adhya, Gurdeep Rastogi, Ajit K Pattnaik, Vishkha Raina Draft genome sequence of Pseudomonas sp. strain BMS12, a plant growth promoting and protease producing bacterium isolated from the rhizosphere sediment of Phragmites karka of Chilika lake, doi: 10.1128/genomeA.00342-16

• A.N.Panda, Samir R. Mishra, Lopamudra Ray, Neha Sahu, Ankita Acharya, Sudhir Jadhao, Mrutyunjay Suar, Tapan K Adhya, Gurdeep Rastogi, Ajit k Pattnayak, Vishakha Raina. Draft genome sequence of Halobacillus sp. strain KGW1, a moderately halophilic and alkaline protease producing bacterium isolated from the rhizospheric region of phragmites karka from Chilika lake, odisha India. doi: 10.1128/genomeA.00361-16.

• Ananta N. Panda1, Samir Ranjan Mishra1, Lopamudra Ray1,4, Surajit Das3,Gurdeep Rastogi2, Ajit Kumar Pattanaik2, Tapan Kumar Adhya1, Mrutyunjay Suar1, Vishakha raina. Taxonomic description and genome sequence of Halobacillus marinus sp. nov.,a novel strain isolated from Chilika Lake, India. doi: 10.1007/s12275-018-7387-x.

THESIS:

DOCTOR OF PHILOSOPHY IN BIOTECHNOLOGY.

 Title: Isolation , taxonomic characterization, partial purification of chitinases and application potential of novel Streptomyces strains from Chilika lake

Description: Use of various classical and modern microbiological tool for the taxonomic identification of the microorganism and purification strategies for the isolation of the enzyme.

Supervisor: Dr. Vishakha Raina, School of Biotechnology, KIIT University.

Duration: 2007 (Dec)- 2012 (August)

MASTERS OF SCIENCES IN MICROBIOLOGY

Title: Microbial community function analysis in anoxic rice soil under long term Nutrient management practice.

Description: Use of different tests including methane emission by GC to analyze the function of the microbial community in anoxic rice soil.

Supervisor: Dr. B. Ramakrishnan, Central Rice Research Institute

Duration: 8 months (April 2003- October 2003).

PROFESSIONAL EXPERIENCES:

  1. Teaching as an Assistant ProfessorAnnie Besant Women’s College (Osmania University, Hyderabad) (April ’07-Nov’2007)  Environmental Microbiology, Basic Microbiology, Molecular Biology
  2. Working as a Junior research fellowat Agharkar Research Institute (DST), Pune for 1 year( September’ 05-September 06)
  3. Teaching as a lecturerat Trident Academy of Technolgy, Bhubaneswar,Orissa (Utkal University) (Environmental Microbiology, Molecular Biology)
  4. Teaching as a lecturerat MITS, Rayagada, Orissa , India 6 months(March ’05-August 05) (Berhampur University) (Environmental Microbiology, Industrial Microbiology)

TECHNICAL AND RESEARCH SKILLS:

Microbiology:Basic and modern microbiological methods for isolation, identification and characterization of microorganisms from various habitat, especially Streptomyces

Molecular Biology: Soil DNA isolation,Genomic & plasmid DNA isolation from Prokaryotes, Gene Cloning and sequencing in prokaryotic system, PCR, Gel Electrophoresis, Restriction digestion of plasmid DNA, Southern Hybridization

Enzymology:Purification of chitinase enzyme from prokaryotic system (Streptomyces) using various column chromatographic techniques.

Chromatography: Thin Layer Chromatography (TLC), Column Chromatography, Gas Liquid Chromatography (GLC), Gas Chromatography-Mass Spectroscopy (GC-MS), and Nuclear Magnetic Resonance (NMR) for the identification of epsilon HCH isomer and its metabolites.

Scientific Computer Software:Phylogenetic analysis (PHYLIP, MEGA and Eztaxon), ClustalX for Multiple sequence alignment, ClustalW, BioEdit, Sequence analyzer software for assemble the nucleotide sequence, RDP–Ribosomal Database project software, End Note, MS-word, PowerPoint and Excel .

Overseas Work Experience:

Worked as a trainee for six months in the laboratory of Dr. Hans Peter Kohler & Dr. Thomas Poiger (july-October, 2010) at Eawag (UMIK, Swiss Federal Institute of Aquatic Science and Technology), Department of Environmental Microbiology, CH-8600, Dubendorf, Switzerland & Research station Agroscope Changins-Wadenswil, Plant Protection Chemistry, Switzerland in the project entitled “Environmental Risk and Bioremediation of Hexachlorocyclohexane Isomers” under the “Indo Swiss Collaboration in Biotechnology (ISCB)”.

LIST OF PUBLICATIONS

  • Lopamudra Ray, Mrutyunjay Suar, Ajit Kumar Pattnaik, Vishakha Raina. Streptomyces chilikensis nov., a novel halophilic streptomycete isolated from brackish water sediment of Lake Chilika in Odisha (IJSEM 2013), 63, 2757–2764
  •  Lopamudra Ray, Samir Ranjan Mishra, Ananta Narayan Panda,  Gurdeep Rastogi, Ajit Kumar Pattanaik, Tapan Kumar Adhya, Mrutyunjay Suar  And Vishakha Raina

Streptomyces barkulensis sp. nov., a novel halophilic streptomycete isolated from an estuarine lake  (IJSEM2014), 64, 1365–1372)

 N.Panda, Samir R. Mishra, Lopamudra Ray, Neha Sahu, Ankita Acharya, Sudhir Jadhao, Mrutyunjay Suar, Tapan K Adhya, Gurdeep Rastogi, Ajit k Pattnayak, Vishakha Raina

Draft genome sequence of Halobacillus sp. strain KGW1, a moderately halophilic and alkaline protease producing bacterium isolated from the rhizospheric region of phragmites karka from Chilika lake, odisha India (Genome Announcement, 2016).

Lopamudra Ray, Samir Ranjan Mishra,Ananta Narayan Panda,  Gurdeep Rastogi, Ajit Kumar Pattanaik,2Tapan Kumar Adhya,Mrutyunjay Suar1and Vishakha Raina Streptomyces chitinivorans sp. Nov.,  a chitinolytic  strain  isolated from estuarine chilika lake sediment in Odisha, india. IJSEM 2016

  • Samir R. Mishra, Lopamudra Ray, A.N.Panda, Neha Sahu, Sonal S. Xess, Sudhir Jadhao, Mrutyunjay Suar, Tapan K Adhya, Gurdeep Rastogi, Ajit K. Pattnayak, Vishakha Raina.

Draft genome sequence of Acinetobacter sp. strain BMW17, a cellulytic and plant growth promoting bacterium isolated from the rhizospheric region of Phragmites karka of Chilika lake, india .Genome Announcment 2016

  • Samir R. Mishra, A.N. Panda, Lopamudra Ray, Neha Sahu, Gayatri Mishra, Sudhir Jadhao, Mrutyunjy Suar, Tapan K Adhya, Gurdeep Rastogi, Ajit K Pattnaik, Vishkha Raina

Draft genome sequence of Pseudomonas sp. strain BMS12, a plant growth promoting and protease producing bacterium isolated from the rhizosphere sediment of Phragmites karka of Chilika lake, India. Genome Announcment 2016

ACCOLADES:

1.Qualified National Eligibility Test conducted by Indian Council of Agricultural Research 2005

2.Attained distinction with Zoology (Hon’s) in Bachelor of Sciences.

Oral Presentataion:

  1. Ramakrishnan B.,Sinha S., Ray Lopamudra ., Samantray R.N., Mishra A.K., Rao V.R. (2004).  Temperature dependence of methane production in anoxic rice soil under longterm monocropping management (Oral presentation) pp 25-26: In Indian Environment Congress 2004, Thiruvanantpuram.
  1. Lopamudra Ray, Malla Padma, Dibya Bhol, Samir ranjan Mishra, A.N. Panda, Gurdeep Rastogi, T.K. Adhya,  Ajit Kumar Pattnaik, Mrutyunjay Suar, Vishakha

Raina (2015) Partial purification and characterization of a low molecular weight and industrially important chitinase enzyme from Streptomyces chilikensis RC1830, a novel strain isolated from Chilika Lake, India. ICEIAM 2015: XIII International

Conference on Environmental, Industrial and Applied Microbiology, January22-24, Paris, France

 Posters presented in Conferences:

1.Ramakrishnan B., Ray Lopamudra.,Samantray R.N.,Mishra A.K.,Rao V.R(2004).  Methanogenic potential of anoxic rice soil under long term nutrient management in tropical India(Abstract selected for poster presentataion in World Rice Research conference, Tokyo, November 4-7, 2004.

2.Ramakrishnan B., Prusty R., Ray Lopamudra., ., Samantray R.N., Mishra A.K.,Rao V.R.(2005). Structural composition of microbial communities in anoxic rice soil under intensive rice cultivation of tropical India .In: Microbial Diversity 2005. International Conference-Microbial Diversity: Current perspective and potential application. April 16-18, 2005, New Delhi.

3.Lopamudra Ray, R.M Rathore,  P. Luethy, M.Suar,  J.S.Purohit ,Vishakha Raina. A Novel Producer of Chitinase from a unique habitat of Orissa : Applications and Prospective. AMI Conference 2008
4.Lopamudra Ray,  R.M Rathore,  P. Luethy, M.Suar,  J.S.Purohit ,Vishakha Raina A study of chitinolytic actinomycete from chilika lake of  orissa, india AMI Conference 2009
5.Lopamudra Ray, Thomas Poiger, Vishakha Raina Residues of  Hexachlorocyclohexane isomers and  metabolites in the agricultural and Industrial soil and water samples of Orissa

6.Lopamudra Ray, Purusottam Mohapatra, RanjanPreet, Chanakyanath Kundu,Vishakha Raina.  Residues  of Hexachlorocyclohexane isomers and metabolites in the agricultural / Industrial soil and water samples of Orissa and evaluation of the toxicity of these compounds in Human Breast Cancer Cell line.

7.Lopamudra Ray, Swatishree Padhi,  Thomas Poiger, Birendranath Banerjee, Vishakha Raina. Residues of Hexachlorocyclohexane isomers and their degadation metabolites in the agricultural and Industrial soil samples of Odisha  and the Evaluation of genotoxicty of Beta-HCH and its degradation metabolite by micronuclei assay.

8.Sameer R. Mishra,  Anant N.Panda, Aditya Dash, Timothy M. Vogel, Lopamudra Ray, Luna Goswami, Vishakha Raina,Ajit k. Pattnaik, Mrutyunjay Suar.

Culturable and Metagenomic approaches to  find novel Bioactive compounds from Chilika Lake: The Future bioprospecting destination .

9.Lopamudra Ray , R.M.Rathore, Sameer R. Mishra, Sabyasachi Dash, Ananta N. Panda, Aditya Dash,  Vishakha Raina, Luna Goswami,  Archana Ghatak, Ajit K. Pattanaik, Mrutyunjay Suar.

An investigation of novel multi-enzyme producing microorganisms from Chilika Lake: Study of a unique chitinase producing Streptomyces.